REPELLENTS IN CRYPTOMERIA JAPONICA AGAINST ARMADILLIDIUMVULGARE.

Chul-Sa Kim¹, Jun Morisawa¹, Michio Horiike¹and Chisato Hirano^2
1Kochi University, Nankoku 738, Japan.
²Nagano University, Ueda, 386-06 Japan.

During studies on components in Cryptomeria japonica, we foundthat the crude methanol extract of C. japonica strongly repelledone species of isopods, Armadillidium vulgare, which is well knownas an unpleasant insect in a house or pest of vegetables in Japan. Thecrude methanol extract suspended in water was successively extracted withhexane, ether, ethyl acetate and n-butanol. The active ether-soluble fractionwas then chromatographed on a silica gel column, eluting it in sequencewith a mixture of increasing concentrations of ether in hexane. Of thoseobtained fractions, the 30% of ether in hexane fraction was most active.This fraction was further separated into four fractions by using HPLC.The bioassay on the HPLC fractions revealed that the activity was not dueto a single component but plural components, because each separated fractiondid not show any activity against A. vulgare and the activity wasrecovered only when the two fractions were combined. From these activetwo fractions, two compounds were isolated and identified as sandaracopimarinoland (+)-10-bisabolatrien-1-ol-4-one, respectively, from their NMR, MS andIR spectra. These compounds repelled A. vulgare only when they werecombined.