p-118

(p-118)NON-HOST SIGNALS INHIBITING FEEDING IN Hylobius PINE WEEVILS: LABORATORY ASSAYS AND FIELD TESTS

Fredrik Schlyter

Chemical Ecology, Plant Protection Science, SLU, P.O. Box 44, 230 53 Alnarp, Sweden.


Anti-feedants as a protection against herbivores have been described in many plants, well-known is the 'neem' tree Azadiractica indica. In conifers defense is quantitative, based on less toxic oleoresin compounds. In small, stressed conifers like the ones planted on clear-cuts in forestry, the quantitative defense is weak. These seedlings have been defended by insecticides like DDT and pyrethroids against the feeding by pine weevils Hylobius abietis (causing a 100 million US$/year damage in Sweden), but use of insecticides will be banned in 2003.
Out work follows two lines: 1) The screening of synthetically available anti-feedant compounds and related structures. 2) The search for antifeedants in non-host plants. The development of laboratory bioassays has been a prerequisite for the project. Initially, we modified a twig technique of Salom et al. (1994). Later, to test small amounts of synthetics and extracts, we developed a micro feeding assay using TLC-plates. Presently, we are modifying an olfactometer to investigate the mode of action (taste/smell) of synthetics. Field tests in 1997 and 1998 demonstrate that formulated feeding deterrents may protect seedlings for months. The long-term formulation of antifeedants, their mode of action, and finding of lower volatility compounds remain our goals.
Klepzig, K., & Schlyter, F. 1999. Laboratory evaluation of plant derived antifeedants against pine weevil Hylobius abietis twig feeding. - J. Econ. Entomol. 92:644-650.


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